IN VITRO ANTI-NEUROINFLAMMATORY EFFECT OF GENISTEIN (4',5,7-TRIHYDROXYISOFLAVONE) ON MICROGLIA HMC3 CELL LINE, AND IN SILICO EVALUATION OF ITS INTERACTION WITH ESTROGEN RECEPTOR-β

Objective: This study was aimed to evaluate the role of genistein or 4',5,7-trihydroxyisoflavone as a phytoestrogen in treatment estrogen deficiency-induced neuroinflammatory. The specific objectives this were determine anti-neuroinflammatory effect through measurement MHC II and Arg1 expressions on microglia HMC3 cell line, well prove that occurs an ER-dependent manner, free-ERβ expression.
 Methods: cells cultured 24-well microplates, induced with 10 ng IFN-γ, incubated for 24 h activate M1 phenotype which has pro-inflammatory characteristics. Genistein concentration 50 μM added cells. expression II, Arg1, markers tested immunocytochemistry method measured using CLSM instrument. In silico approach also conducted interaction between ERβ, compared 17β-estradiol. structure prepared Avogadro 1.0.1, molecular docking done PyRx 0.8 software. Biovia Discovery Studio Visualizer 2016 used visualize against 3OLS protein. physicochemical characteristics analyzed SwissADME web tool.
 Results: can decrease increase negative controls (p<0.005), value 472.577±26.701 AU 114.299±6.578 AU. But, cannot (p<0.005). results analysis showed is ERβ agonist.
 Conclusion: shows effects by decreasing increasing line. However, does not occur binding but it likely other types ER.